ARG59827
anti-EIF2AK4 / GCN2 antibody
anti-EIF2AK4 / GCN2 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes EIF2AK4 / GCN2 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | EIF2AK4 / GCN2 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-300 of Human EIF2AK4 (NP_001013725.2). |
| Conjugation | Un-conjugated |
| Alternate Names | EC 2.7.11.1; GCN2; GCN2-like protein; PVOD2; Eukaryotic translation initiation factor 2-alpha kinase 4 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | HepG2 | ||||||||
| Observed Size | 187 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9P2K8 Human Eukaryotic translation initiation factor 2-alpha kinase 4 Swiss-port # Q9QZ05 Mouse Eukaryotic translation initiation factor 2-alpha kinase 4 |
|---|---|
| Gene Symbol | EIF2AK4 |
| Gene Full Name | eukaryotic translation initiation factor 2 alpha kinase 4 |
| Background | This gene encodes a member of a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 (EIF2), resulting in the downregulaton of protein synthesis. The encoded protein responds to amino acid deprivation by binding uncharged transfer RNAs. It may also be activated by glucose deprivation and viral infection. Mutations in this gene have been found in individuals suffering from autosomal recessive pulmonary venoocclusive-disease-2. [provided by RefSeq, Mar 2014] |
| Function | Can phosphorylate the alpha subunit of EIF2 and may mediate translational control. [UniProt] |
| Cellular Localization | Cytoplasm. [UniProt] |
| Calculated MW | 187 kDa |
| PTM | Autophosphorylated; autophosphorylation on Thr-899 is increased upon amino acid starvation and in UV irradiation cells and inhibited in presence of IMPACT. [UniProt] |
