ARG59715
anti-EIF2AK4 / GCN2 phospho (Thr899) antibody
anti-EIF2AK4 / GCN2 phospho (Thr899) antibody for Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes EIF2AK4 / GCN2 phospho (Thr899) |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Specificity | Recognizes endogenous levels of EIF2AK4 / GCN2 phospho (Thr899) protein. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | EIF2AK4 / GCN2 |
| Antigen Species | Human |
| Immunogen | Phosphospecific peptide around Thr899 of Human EIF2AK4 / GCN2. |
| Conjugation | Un-conjugated |
| Alternate Names | EC 2.7.11.1; GCN2; GCN2-like protein; PVOD2; Eukaryotic translation initiation factor 2-alpha kinase 4 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Observed Size | ~ 250 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9P2K8 Human Eukaryotic translation initiation factor 2-alpha kinase 4 |
|---|---|
| Gene Symbol | EIF2AK4 |
| Gene Full Name | eukaryotic translation initiation factor 2 alpha kinase 4 |
| Background | This gene encodes a member of a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 (EIF2), resulting in the downregulaton of protein synthesis. The encoded protein responds to amino acid deprivation by binding uncharged transfer RNAs. It may also be activated by glucose deprivation and viral infection. Mutations in this gene have been found in individuals suffering from autosomal recessive pulmonary venoocclusive-disease-2. [provided by RefSeq, Mar 2014] |
| Function | Can phosphorylate the alpha subunit of EIF2 and may mediate translational control. [UniProt] |
| Cellular Localization | Cytoplasm. [UniProt] |
| Calculated MW | 187 kDa |
| PTM | Autophosphorylated; autophosphorylation on Thr-899 is increased upon amino acid starvation and in UV irradiation cells and inhibited in presence of IMPACT. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG59715 anti-EIF2AK4 / GCN2 phospho (Thr899) antibody WB image
Western blot: HeLa cells untreated (left) or treated with Calyculin (right). Cell lysates were stained with ARG59715 anti-EIF2AK4 / GCN2 phospho (Thr899) antibody.
