ARG55553
anti-EMR1 F4/80 antibody [BM8]
anti-EMR1 F4/80 antibody [BM8] for Flow cytometry,IHC-Frozen sections,Western blot and Human,Mouse
Mouse Inflammatory Cell Marker antibody; Macrophage Marker antibody
Overview
| Product Description | Rat Monoclonal antibody [BM8] recognizes EMR1 F4/80 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | FACS, IHC-Fr, WB |
| Specificity | Recognizes a 125 kDa of mouse extracellular macrophage membrane molecule, highly restricted to mature macrophage subpopulations residing in tissue. Species Crossreactivity: human heart macrophages. |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | BM8 |
| Isotype | IgG2a |
| Target Name | EMR1 F4/80 |
| Antigen Species | Mouse |
| Immunogen | BALB/c macrophages obtained from 14 day old bone marrow cell cultures. |
| Conjugation | Un-conjugated |
| Alternate Names | EGF-like module-containing mucin-like hormone receptor-like 1; EMR1 hormone receptor; Emr1; DD7A5-7; Adhesion G protein-coupled receptor E1; Ly71; EGF-like module receptor 1; Gpf480; F4/80; Cell surface glycoprotein F4/80; EGF-TM7; TM7LN3 |
Application Instructions
| Application Suggestion |
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| Application Note | WB: 1:50 detects F4/80 in mouse bone marrow derived macrophages under non-reduced conditions. Reduction with 2-mercaptoethanol destroys BM8 antigen. Flow Cytometry: Fix with 1% paraformaldehyde. IHC-Fr: Tissue embedded in OCT Tissue Tec, fixed with acetone for 10 minutes at RT. Incubate with PBS, 0.02M sodium azide, 0.1% H2O2 for 10 minutes at RT to destroy endogenous peroxidase. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | IHC-Fr: Spleen |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS, 0.02% Sodium azide and 0.1% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.1% BSA |
| Concentration | 0.1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q14246 Human Adhesion G protein-coupled receptor E1 Swiss-port # Q61549 Mouse Adhesion G protein-coupled receptor E1 |
|---|---|
| Gene Symbol | Adgre1 |
| Gene Full Name | adhesion G protein-coupled receptor E1 |
| Background | EMR1 F4/80 has a domain resembling seven transmembrane G protein-coupled hormone receptors (7TM receptors) at its C-terminus. The N-terminus of the encoded protein has six EGF-like modules, separated from the transmembrane segments by a serine/threonine-rich domain, a feature reminiscent of mucin-like, single-span, integral membrane glycoproteins with adhesive properties. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012] |
| Function | EMR1 F4/80: Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development. [UniProt] |
| Highlight | Related products: EMR1 F4/80 antibodies; EMR1 F4/80 Duos / Panels; Anti-Rat IgG secondary antibodies; Related news: Exploring Antiviral Immune Response |
| Research Area | Mouse Inflammatory Cell Marker antibody; Macrophage Marker antibody |
| Calculated MW | 98 kDa |
