ARG23155

anti-EMR3 antibody [3D7] (FITC)

anti-EMR3 antibody [3D7] (FITC) for Flow cytometry and Human

Overview

Product Description FITC-conjugated Hamster Monoclonal antibody [3D7] recognizes EMR3
Hamster anti Human EMR3 antibody, clone 3D7 recognizes human epidermal growth factor (EGF) module-containing mucin-like hormone receptor 3 (EMR3), a ~56 kDa member of the EGF-7 transmembrane (TM7) family of adhesion receptors. EMR3 is expressed at the cell surface as a heterodimer. The molecule is predominantly expressed on granulocytes, and at lower levels on mature myeloid cells, monocytes and dendritic cells. EMR3 is absent on lymphocytes, haematopoietic stem cells and myeloid progenitors. Studies suggest that the EMR3 molecule is up-regulated during late stages of neutrophil differentiation and is a marker for terminally differentiated cells. The exact functions of EMR3 and its ligands have not yet been determined.
Tested Reactivity Hu
Tested Application FACS
Host Hamster
Clonality Monoclonal
Clone 3D7
Isotype IgG
Target Name EMR3
Antigen Species Human
Immunogen ARHO-EMR3-CD97 (EGF1) transfectants.
Conjugation FITC
Alternate Names EGF-like module receptor 3; EMR3; Adhesion G protein-coupled receptor E3; EGF-like module-containing mucin-like hormone receptor-like 3

Application Instructions

Application Suggestion
Tested Application Dilution
FACSNeat - 1:10
Application Note FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS and 0.09% Sodium azide.
Preservative 0.09% Sodium azide
Concentration 0.1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 84658 Human ADGRE3

Swiss-port # Q9BY15 Human Adhesion G protein-coupled receptor E3

Gene Symbol ADGRE3
Gene Full Name adhesion G protein-coupled receptor E3
Background This gene encodes a member of the class B seven-span transmembrane (TM7) receptor family expressed predominantly by cells of the immune system. Family members are characterized by an extended extracellular region with a variable number of N-terminal epidermal growth factor (EGF)-like domains coupled to a TM7 domain via a mucin-like spacer domain. This gene is closely linked to the gene encoding egf-like molecule containing mucin-like hormone receptor 2 on chromosome 19. This protein may play a role in myeloid-myeloid interactions during immune and inflammatory responses. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2014]
Function Orphan receptor that may play a role myeloid-myeloid interactions during immune and inflammatory responses. A ligand for the soluble form of this receptor is present at the surface of momocytes-derived macrophages and activated neutrophils. [UniProt]
Calculated MW 73 kDa
PTM Proteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane subunit. [UniProt]