ARG42135

anti-ENC1 antibody

anti-ENC1 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human,Rat

Overview

Product Description Goat Polyclonal antibody recognizes ENC1
Tested Reactivity Hu, Rat
Predict Reactivity Ms, Cow, Dog, Pig
Tested Application IHC-P, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name ENC1
Antigen Species Human
Immunogen Synthetic peptide around the internal region of Human ENC1. (TSVSHDKLPKVQC) (NP_003624.1; NP_001243505.1)
Conjugation Un-conjugated
Alternate Names KLHL37; KLHL35; Kelch-like protein 37; TP53I10; PIG10; NRPB; Nuclear matrix protein NRP/B; CCL28; ENC-1; Ectoderm-neural cortex protein 1; p53-induced gene 10 protein

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P5 µg/ml
WB1 - 3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Rat ovary
Observed Size ~ 70 kDa

Properties

Form Liquid
Purification Ammonium sulphate precipitation followed by affinity purification with immunogen.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 8507 Human ENC1

Swiss-port # O14682 Human Ectoderm-neural cortex protein 1

Gene Symbol ENC1
Gene Full Name ectodermal-neural cortex 1 (with BTB domain)
Background This gene encodes a member of the kelch-related family of actin-binding proteins. The encoded protein plays a role in the oxidative stress response as a regulator of the transcription factor Nrf2, and expression of this gene may play a role in malignant transformation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012]
Function Actin-binding protein involved in the regulation of neuronal process formation and in differentiation of neural crest cells. Down-regulates transcription factor NF2L2/NRF2 by decreasing the rate of protein synthesis and not via a ubiquitin-mediated proteasomal degradation mechanism. [UniProt]
Cellular Localization Nucleus matrix. Cytoplasm. Cytoplasm, cytoskeleton. [UniProt]
Calculated MW 66 kDa
PTM Ubiquitinated by E3 ubiquitin ligase complex formed by CUL3 and RBX1 and probably targeted for proteasome-independent degradation. Quinone-induced oxidative stress increases its ubiquitination. [UniProt]