ARG55574

anti-ENO3 antibody

anti-ENO3 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Cancer antibody; Metabolism antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes ENO3
Tested Reactivity Hu
Predict Reactivity Ms, Rat, Bov, Pig
Tested Application FACS, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name ENO3
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 237-264 (Center) of Human ENO3.
Conjugation Un-conjugated
Alternate Names Skeletal muscle enolase; Muscle-specific enolase; 2-phospho-D-glycerate hydro-lyase; Enolase 3; Beta-enolase; MSE; GSD13; EC 4.2.1.11

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:10 - 1:50
IHC-P1:50 - 1:100
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse skeletal muscle

Properties

Form Liquid
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2027 Human ENO3

Swiss-port # P13929 Human Beta-enolase

Gene Symbol ENO3
Gene Full Name enolase 3 (beta, muscle)
Background This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme is found in skeletal muscle cells in the adult where it may play a role in muscle development and regeneration. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene have be associated glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Jul 2010]
Function Appears to have a function in striated muscle development and regeneration. [UniProt]
Cellular Localization Cytoplasm. Note=Localized to the Z line. Some colocalization with CKM at M-band (By similarity)
Research Area Cancer antibody; Metabolism antibody; Signaling Transduction antibody
Calculated MW 47 kDa