ARG22202
anti-ENaC gamma antibody
anti-ENaC gamma antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Hamster,Xenopus laevis
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ENaC gamma |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Hm, Xenopus laevis |
| Tested Application | ICC/IF, IHC-P, WB |
| Specificity | Detects ~83kDa. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ENaC gamma |
| Antigen Species | Rat |
| Immunogen | Synthetic peptide around aa. 629-650 (C-terminus) of Rat ENaC Gamma |
| Conjugation | Un-conjugated |
| Alternate Names | ENaCG; Epithelial Na; Gamma-NaCH; Gamma-ENaC; ENaCg; PHA1; SCNEG; BESC3; ENaCgamma; Amiloride-sensitive sodium channel subunit gamma; Nonvoltage-gated sodium channel 1 subunit gamma |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS, 0.09% Sodium azide and 50% Glycerol |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Scnn1g |
| Gene Full Name | sodium channel, non-voltage-gated 1, gamma subunit |
| Background | Nonvoltage-gated, amiloride-sensitive, sodium channels control fluid and electrolyte transport across epithelia in many organs. These channels are heteromeric complexes consisting of 3 subunits: alpha, beta, and gamma. This gene encodes the gamma subunit, and mutations in this gene have been associated with Liddle syndrome. [provided by RefSeq, Apr 2009] |
| Function | Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception. [UniProt] |
| Cellular Localization | Apical cell membrane |
| Calculated MW | 74 kDa |
| PTM | Phosphorylated on serine and threonine residues. Aldosterone and insulin increase the basal level of phosphorylation. Ubiquitinated; this targets individual subunits for endocytosis and proteasome-mediated degradation. ENaC cleavage by furin, and subsequently by prostasin (PRSS8), leads to a stepwise increase in the open probability of the channel as a result of release of the alpha and gamma subunit inhibitory tracts, respectively. Interaction of ENaC subunit SCNN1B with BPIFA1 protects ENaC against proteolytic activation. |
