ARG22660

anti-ER-MP58 antibody [ER-MP58]

anti-ER-MP58 antibody [ER-MP58] for Flow cytometry,ICC/IF,IHC-Frozen sections,Immunoprecipitation and Mouse

Overview

Product Description Rat Monoclonal antibody [ER-MP58] recognizes ER-MP58
This antibody recognizes murine antigen ER-MP58, which is expressed by all bone marrow-derived M-CSF- and GM-CSF-responsive myeloid blood cell precursors.
The expression of ER-MP58 remains at a high level throughout the precursor/monocyte stage and is down-regulated upon maturation into mature macrophages. The ER-MP58 antigen is used to distinguish between early myeloid-committed cells and other haematopoietic progenitors cells in the BM. The antigen has been used as a marker of murine macrophage development in the BM.
ER-MP58 is suitable for the identification of myeloid haemopoietic islands in various organs, and for embryonal studies.
Tested Reactivity Ms
Tested Application FACS, ICC/IF, IHC-Fr, IP
Host Rat
Clonality Monoclonal
Clone ER-MP58
Isotype IgG2a
Target Name ER-MP58
Antigen Species Mouse
Immunogen Balb/c macrophage precursor cell hybrids
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
FACSAssay-dependent
ICC/IFAssay-dependent
IHC-Fr1:25 - 1:100
IPAssay-dependent
Application Note FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS and 0.09% Sodium azide.
Preservative 0.09% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.