ARG51579
anti-ERBB2 / HER2 phospho (Tyr1221 / Tyr1222) antibody
anti-ERBB2 / HER2 phospho (Tyr1221 / Tyr1222) antibody for Western blot,ICC/IF and Human
Cancer antibody; Controls and Markers antibody; Signaling Transduction antibody; Circulating Tumor Cells BioMarker antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ERBB2 / HER2 phospho (Tyr1221 / Tyr1222) |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ERBB2 / HER2 |
| Antigen Species | Human |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine1221/1222 (N-L-Y(p)-Y(p)-W) derived from Human HER2. |
| Conjugation | Un-conjugated |
| Alternate Names | p185erbB2; Proto-oncogene c-ErbB-2; Metastatic lymph node gene 19 protein; Proto-oncogene Neu; NGL; EC 2.7.10.1; CD340; CD antigen CD340; TKR1; HER-2; Tyrosine kinase-type cell surface receptor HER2; HER2; NEU; HER-2/neu; MLN 19; Receptor tyrosine-protein kinase erbB-2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Buffer | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P04626 Human Receptor tyrosine-protein kinase erbB-2 |
|---|---|
| Gene Symbol | ERBB2 |
| Gene Full Name | erb-b2 receptor tyrosine kinase 2 |
| Background | Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF-alpha and amphiregulin. |
| Function | Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth. [UniProt] |
| Highlight | Related Antibody Duos and Panels: ARG30034 Phospho ERBB2 / HER2 Antibody Duo (Total, pY1221/1222) Related products: ERBB2 antibodies; ERBB2 ELISA Kits; ERBB2 Duos / Panels; Anti-Rabbit IgG secondary antibodies; |
| Research Area | Cancer antibody; Controls and Markers antibody; Signaling Transduction antibody; Circulating Tumor Cells BioMarker antibody |
| Calculated MW | 138 kDa |
| PTM | Autophosphorylated. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit (Probable). Ligand-binding increases phosphorylation on tyrosine residues (PubMed:27134172). Signaling via SEMA4C promotes phosphorylation at Tyr-1248 (PubMed:17554007). Dephosphorylated by PTPN12 (PubMed:27134172). |
