ARG54172

anti-ERCC1 antibody

anti-ERCC1 antibody for Western blot and Human

Cancer antibody; Gene Regulation antibody
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Overview

Product Description Mouse Monoclonal antibody recognizes ERCC1
Tested Reactivity Hu
Tested Application WB
Host Mouse
Clonality Monoclonal
Isotype IgG2b
Target Name ERCC1
Antigen Species Human
Immunogen Purified recombinant human ERCC1 protein fragments expressed in E.coli.
Conjugation Un-conjugated
Alternate Names DNA excision repair protein ERCC-1; RAD10; COFS4; UV20

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 39 kDa

Properties

Form Liquid
Purification Affinity purified
Buffer PBS (pH 7.4), 0.02% Sodium azide, 0.1mg/ml BSA and 50% Glycerol
Preservative 0.02% Sodium azide
Stabilizer 0.1mg/ml BSA, 50% Glycerol
Concentration 18 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2067 Human ERCC1

Swiss-port # P07992 Human DNA excision repair protein ERCC-1

Gene Symbol ERCC1
Gene Full Name excision repair cross-complementation group 1
Background Isoform 1:Non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair.Responsible,in conjunction with SLX4,for the first step in the repair of interstrand cross-links (ICL).Participates in the processing of anaphase bridge-generating DNA structures,which consist in incompletely processed DNA lesions arising during S or G2 phase,and can result in cytokinesis failure.Also required for homology-directed repair (HDR) of DNA double-strand breaks,in conjunction with SLX4.
Function Isoform 1: Non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. Responsible, in conjunction with SLX4, for the first step in the repair of interstrand cross-links (ICL). Participates in the processing of anaphase bridge-generating DNA structures, which consist in incompletely processed DNA lesions arising during S or G2 phase, and can result in cytokinesis failure. Also required for homology-directed repair (HDR) of DNA double-strand breaks, in conjunction with SLX4. [UniProt]
Cellular Localization Isoform 1: Nucleus. Isoform 2: Cytoplasm. Nucleus. Isoform 3: Nucleus. Isoform 4: Nucleus.
Research Area Cancer antibody; Gene Regulation antibody
Calculated MW 33 kDa

Customer's Feedback

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Review for anti-ERCC1 antibody

Application:WB

Sample:MCF7 and 293T

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC