ARG54172
anti-ERCC1 antibody
anti-ERCC1 antibody for Western blot and Human
Cancer antibody; Gene Regulation antibody
1
Overview
| Product Description | Mouse Monoclonal antibody recognizes ERCC1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Target Name | ERCC1 |
| Antigen Species | Human |
| Immunogen | Purified recombinant human ERCC1 protein fragments expressed in E.coli. |
| Conjugation | Un-conjugated |
| Alternate Names | DNA excision repair protein ERCC-1; RAD10; COFS4; UV20 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Observed Size | 39 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide, 0.1mg/ml BSA and 50% Glycerol |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.1mg/ml BSA, 50% Glycerol |
| Concentration | 18 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P07992 Human DNA excision repair protein ERCC-1 |
|---|---|
| Gene Symbol | ERCC1 |
| Gene Full Name | excision repair cross-complementation group 1 |
| Background | Isoform 1:Non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair.Responsible,in conjunction with SLX4,for the first step in the repair of interstrand cross-links (ICL).Participates in the processing of anaphase bridge-generating DNA structures,which consist in incompletely processed DNA lesions arising during S or G2 phase,and can result in cytokinesis failure.Also required for homology-directed repair (HDR) of DNA double-strand breaks,in conjunction with SLX4. |
| Function | Isoform 1: Non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. Responsible, in conjunction with SLX4, for the first step in the repair of interstrand cross-links (ICL). Participates in the processing of anaphase bridge-generating DNA structures, which consist in incompletely processed DNA lesions arising during S or G2 phase, and can result in cytokinesis failure. Also required for homology-directed repair (HDR) of DNA double-strand breaks, in conjunction with SLX4. [UniProt] |
| Cellular Localization | Isoform 1: Nucleus. Isoform 2: Cytoplasm. Nucleus. Isoform 3: Nucleus. Isoform 4: Nucleus. |
| Research Area | Cancer antibody; Gene Regulation antibody |
| Calculated MW | 33 kDa |
Customer's Feedback
Good
Review for anti-ERCC1 antibody
Application：WB
Sample：MCF7 and 293T
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
