ARG57839

anti-ERCC1 antibody

anti-ERCC1 antibody for Western blot and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes ERCC1
Tested Reactivity Hu, Ms
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name ERCC1
Antigen Species Human
Immunogen Recombinant protein of Human ERCC1.
Conjugation Un-conjugated
Alternate Names DNA excision repair protein ERCC-1; RAD10; COFS4; UV20

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HeLa
Observed Size ~ 38 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 13870 Mouse ERCC1

GeneID: 2067 Human ERCC1

Swiss-port # P07903 Mouse DNA excision repair protein ERCC-1

Swiss-port # P07992 Human DNA excision repair protein ERCC-1

Gene Symbol ERCC1
Gene Full Name excision repair cross-complementation group 1
Background The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand. [provided by RefSeq, Oct 2009]
Function Isoform 1: Non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. Responsible, in conjunction with SLX4, for the first step in the repair of interstrand cross-links (ICL). Participates in the processing of anaphase bridge-generating DNA structures, which consist in incompletely processed DNA lesions arising during S or G2 phase, and can result in cytokinesis failure. Also required for homology-directed repair (HDR) of DNA double-strand breaks, in conjunction with SLX4. [UniProt]
Cellular Localization Cytoplasm, Nucleus. [UniProt]
Calculated MW 33 kDa