ARG64667

anti-ERCC1 antibody

anti-ERCC1 antibody for Western blot and Human

Cancer antibody; Gene Regulation antibody

Overview

Product Description Goat Polyclonal antibody recognizes ERCC1
Tested Reactivity Hu
Tested Application WB
Specificity This antibody is expected to recognise both reported isoforms (NP_973730.1 and NP_001974.1).
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name ERCC1
Antigen Species Human
Immunogen DPGKDKEGVPQPS-C
Conjugation Un-conjugated
Alternate Names DNA excision repair protein ERCC-1; RAD10; COFS4; UV20

Application Instructions

Application Suggestion
Tested Application Dilution
WB0.3 - 1 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2067 Human ERCC1

Swiss-port # P07992 Human DNA excision repair protein ERCC-1

Background The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand. [provided by RefSeq, Oct 2009]
Research Area Cancer antibody; Gene Regulation antibody
Calculated MW 33 kDa