ARG64667
anti-ERCC1 antibody
anti-ERCC1 antibody for Western blot and Human
Cancer antibody; Gene Regulation antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes ERCC1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Specificity | This antibody is expected to recognise both reported isoforms (NP_973730.1 and NP_001974.1). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ERCC1 |
| Antigen Species | Human |
| Immunogen | DPGKDKEGVPQPS-C |
| Conjugation | Un-conjugated |
| Alternate Names | DNA excision repair protein ERCC-1; RAD10; COFS4; UV20 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P07992 Human DNA excision repair protein ERCC-1 |
|---|---|
| Background | The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand. [provided by RefSeq, Oct 2009] |
| Research Area | Cancer antibody; Gene Regulation antibody |
| Calculated MW | 33 kDa |
