ARG43716

anti-ERK3 antibody

anti-ERK3 antibody for IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes ERK3
Tested Reactivity Hu, Ms
Tested Application IHC-P, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name ERK3
Antigen Species Human
Immunogen Synthetic peptide of Human ERK3.
Conjugation Un-conjugated
Protein Full Name Mitogen-activated protein kinase 6
Alternate Names ERK3; HsT17250; p97-MAPK; EC 2.7.11.24; ERK-3; PRKM6; MAP kinase 6; MAP kinase isoform p97; p97MAPK; Mitogen-activated protein kinase 6; Extracellular signal-regulated kinase 3; MAPK 6

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:20 - 1:100
IP1:10 - 1:30
WB1:1000 - 1:5000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control NIH-3T3; HeLa
Observed Size 90-95 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer 50 nM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 50772 Mouse MAPK6

GeneID: 5597 Human MAPK6

Swiss-port # Q16659 Human Mitogen-activated protein kinase 6

Swiss-port # Q61532 Mouse Mitogen-activated protein kinase 6

Gene Symbol MAPK6
Gene Full Name mitogen-activated protein kinase 6
Background The protein encoded by this gene is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters. [provided by RefSeq, Jul 2008]
Function Atypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK3/MAPK6 is phosphorylated at Ser-189 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6. May promote entry in the cell cycle (By similarity). [UniProt]
Cellular Localization Cytoplasm. Nucleus. Translocates to the cytoplasm following interaction with MAPKAPK5. [UniProt]
Calculated MW 83 kDa
PTM Phosphorylated at Ser-189 by PAK1, PAK2 and PAK3 resulting in catalytic activation. Phosphorylated by MAPKAPK5 at other sites.

Ubiquitination at Met-1 leads to degradation by the proteasome pathway. [UniProt]