ARG41859

anti-ERLIN2 / SPFH2 antibody

anti-ERLIN2 / SPFH2 antibody for Western blot and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes ERLIN2 / SPFH2
Tested Reactivity Hu, Ms
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name ERLIN2 / SPFH2
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide between aa. 307-333 of Human ERLIN2 / SPFH2.
Conjugation Un-conjugated
Alternate Names C8orf2; SPFH2; Endoplasmic reticulum lipid raft-associated protein 2; Erlin-2; SPG18; SPFH domain-containing protein 2; Stomatin-prohibitin-flotillin-HflC/K domain-containing protein 2; NET32

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control A431
Observed Size ~ 40 kDa

Properties

Form Liquid
Purification Purification with Protein A and immunogen peptide.
Buffer PBS and 0.09% (W/V) Sodium azide.
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 11160 Human ERLIN2

GeneID: 244373 Mouse ERLIN2

Swiss-port # O94905 Human Erlin-2

Swiss-port # Q8BFZ9 Mouse Erlin-2

Gene Symbol ERLIN2
Gene Full Name ER lipid raft associated 2
Background This gene encodes a member of the SPFH domain-containing family of lipid raft-associated proteins. The encoded protein is localized to lipid rafts of the endoplasmic reticulum and plays a critical role in inositol 1,4,5-trisphosphate (IP3) signaling by mediating ER-associated degradation of activated IP3 receptors. Mutations in this gene are a cause of spastic paraplegia-18 (SPG18). Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012]
Function Component of the ERLIN1/ERLIN2 complex which mediates the endoplasmic reticulum-associated degradation (ERAD) of inositol 1,4,5-trisphosphate receptors (IP3Rs) such as ITPR1. Promotes sterol-accelerated ERAD of HMGCR probably implicating an AMFR/gp78-containing ubiquitin ligase complex. Involved in regulation of cellular cholesterol homeostasis by regulation the SREBP signaling pathway. May promote ER retention of the SCAP-SREBF complex. [UniProt]
Cellular Localization Endoplasmic reticulum membrane; Single-pass type II membrane protein. Note=Associated with lipid raft-like domains of the endoplasmic reticulum membrane. [UniProt]
Calculated MW 38 kDa