ARG63807

anti-ERLIN2 / SPFH2 antibody

anti-ERLIN2 / SPFH2 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Neuroscience antibody; Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes ERLIN2 / SPFH2
Tested Reactivity Hu
Predict Reactivity Rat
Tested Application IHC-P, WB
Specificity This antibody is expected to recognise isoform 1 (NP_009106) only.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name ERLIN2 / SPFH2
Antigen Species Human
Immunogen C-EDEPLETATKEN
Conjugation Un-conjugated
Alternate Names C8orf2; SPFH2; Endoplasmic reticulum lipid raft-associated protein 2; Erlin-2; SPG18; SPFH domain-containing protein 2; Stomatin-prohibitin-flotillin-HflC/K domain-containing protein 2; NET32

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P3 - 5 µg/ml
WB0.3 - 1 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 11160 Human ERLIN2

Swiss-port # O94905 Human Erlin-2

Background This gene encodes a member of the SPFH domain-containing family of lipid raft-associated proteins. The encoded protein is localized to lipid rafts of the endoplasmic reticulum and plays a critical role in inositol 1,4,5-trisphosphate (IP3) signaling by mediating ER-associated degradation of activated IP3 receptors. Mutations in this gene are a cause of spastic paraplegia-18 (SPG18). Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012]
Research Area Neuroscience antibody; Signaling Transduction antibody
Calculated MW 38 kDa