ARG41274

anti-ERVFRD 1 / Syncytin 2 antibody

anti-ERVFRD 1 / Syncytin 2 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes ERVFRD 1 / Syncytin 2
Tested Reactivity Hu
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name ERVFRD 1 / Syncytin 2
Antigen Species Human
Immunogen Recombinant protein corresponding to aa. 16-250 of Human Syncytin 2.
Conjugation Un-conjugated
Alternate Names Syncytin-2; HERV-FRD; envFRD; ERVFRDE1; HERV-W/FRD; GLLL6191; Endogenous retrovirus group FRD member 1; TM; SU; Envelope polyprotein; UNQ6191; HERV-FRD_6p24.1 provirus ancestral Env polyprotein

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:100
WB3 - 5 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 60-70 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 405754 Human ERVFRD-1

Swiss-port # P60508 Human Syncytin-2

Gene Symbol ERVFRD-1
Gene Full Name endogenous retrovirus group FRD, member 1
Background Many different human endogenous retrovirus (HERV) families are expressed in normal placental tissue at high levels, suggesting that HERVs are functionally important in reproduction. This gene is part of a human endogenous retrovirus provirus on chromosome 6 that has inactivating mutations in the gag and pol genes. This gene is the envelope glycoprotein gene which appears to have been selectively preserved. The gene's protein product plays a major role in placental development and trophoblast fusion. The protein has the characteristics of a typical retroviral envelope protein, including a cleavage site that separates the surface (SU) and transmembrane (TM) proteins which form a heterodimer. [provided by RefSeq, Jun 2012]
Function This endogenous retroviral envelope protein has retained its original fusogenic properties and participates in trophoblast fusion and the formation of a syncytium during placenta morphogenesis. The interaction with MFSD2A is apparently important for this process.

Endogenous envelope proteins may have kept, lost or modified their original function during evolution but this one can still make pseudotypes with MLV, HIV-1 or SIV-1 virions and confer infectivity. Retroviral envelope proteins mediate receptor recognition and membrane fusion during early infection. The surface protein mediates receptor recognition, while the transmembrane protein anchors the envelope heterodimer to the viral membrane through one transmembrane domain. The other hydrophobic domain, called fusion peptide, mediates fusion of the viral membrane with the target cell membrane. [UniProt]
Cellular Localization Virion. Surface protein: Cell membrane; Peripheral membrane protein. Note=The surface protein is not anchored to the membrane, but localizes to the extracellular surface through its binding to TM. Transmembrane protein: Cell membrane; Single-pass membrane protein. [UniProt]
Calculated MW 60 kDa
PTM Specific enzymatic cleavages in vivo yield the mature SU and TM proteins.

The CXXC motif is highly conserved across a broad range of retroviral envelope proteins. It is thought to participate in the formation of a labile disulfide bond possibly with the CX6CC motif present in the transmembrane protein. Isomerization of the intersubunit disulfide bond to an SU intrachain disulfide bond is thought to occur upon receptor recognition in order to allow membrane fusion (By similarity). [UniProt]