ARG65147

anti-EVI1 / AML1 antibody

anti-EVI1 / AML1 antibody for Western blot and Human

Gene Regulation antibody

Overview

Product Description Goat Polyclonal antibody recognizes EVI1 / AML1
Tested Reactivity Hu
Tested Application WB
Specificity This antibody is expected to recognize all reported isoforms (NP_001098547.3; NP_005232.2; NP_004982.2; NP_001157471.1; NP_001157472.1). Reported variants represent identical protein: NP_001098548.2, NP_005232.2
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name EVI1 / AML1
Antigen Species Human
Immunogen C-DKESLHSTSH
Conjugation Un-conjugated
Alternate Names MDS1 and EVI1 complex locus protein EVI1; AML1-EVI-1; Ecotropic virus integration site 1 protein homolog; EVI1; MDS1-EVI1; PRDM3; MDS1; EVI-1

Application Instructions

Application Suggestion
Tested Application Dilution
WB1 - 3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2122 Human MECOM

Swiss-port # Q03112 Human MDS1 and EVI1 complex locus protein EVI1

Background The protein encoded by this gene is a transcriptional regulator and oncoprotein that may be involved in hematopoiesis, apoptosis, development, and cell differentiation and proliferation. The encoded protein can interact with CTBP1, SMAD3, CREBBP, KAT2B, MAPK8, and MAPK9. This gene can undergo translocation with the AML1 gene, resulting in overexpression of this gene and the onset of leukemia. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]
Research Area Gene Regulation antibody
Calculated MW 138 kDa
PTM Phosphorylated.
May be acetylated by CREBBP and KAT2B.