ARG58644

anti-EXT1 antibody

anti-EXT1 antibody for Western blot,ICC/IF and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes EXT1
Tested Reactivity Hu, Ms
Tested Application ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name EXT1
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 467-746 of Human EXT1 (NP_000118.2).
Conjugation Un-conjugated
Alternate Names TRPS2; Putative tumor suppressor protein EXT1; LGCR; TTV; EC 2.4.1.224; EXT; Multiple exostoses protein 1; Glucuronosyl-N-acetylglucosaminyl-proteoglycan/N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase; LGS; EC 2.4.1.225; Exostosin-1

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:10 - 1:100
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control PC-3
Observed Size 88 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 14042 Mouse EXT1

GeneID: 2131 Human EXT1

Swiss-port # P97464 Mouse Exostosin-1

Swiss-port # Q16394 Human Exostosin-1

Gene Symbol EXT1
Gene Full Name exostosin glycosyltransferase 1
Background This gene encodes an endoplasmic reticulum-resident type II transmembrane glycosyltransferase involved in the chain elongation step of heparan sulfate biosynthesis. Mutations in this gene cause the type I form of multiple exostoses. [provided by RefSeq, Jul 2008]
Function Glycosyltransferase required for the biosynthesis of heparan-sulfate. The EXT1/EXT2 complex possesses substantially higher glycosyltransferase activity than EXT1 or EXT2 alone. Appears to be a tumor suppressor. [UniProt]
Cellular Localization Endoplasmic reticulum membrane, Single-pass type II membrane protein, Golgi apparatus membrane. [UniProt]
Calculated MW 86 kDa