ARG55271

anti-EZH1 antibody

anti-EZH1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

Gene Regulation antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes EZH1
Tested Reactivity Hu, Ms
Predict Reactivity Rat
Tested Application IHC-P, WB
Specificity Multiple isoforms of EZH1 are known to exist. EZH1 antibody is predicted to not cross-react with EZH2
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name EZH1
Antigen Species Human
Immunogen Synthetic peptide (18 aa) within aa. 180-230 of Human EZH1.
Conjugation Un-conjugated
Alternate Names Enhancer of zeste homolog 1; ENX-2; EC 2.1.1.43; KMT6B; Histone-lysine N-methyltransferase EZH1

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P5 μg/ml
WB1 - 2 μg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse Lung Tissue Lysate

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS and 0.02% Sodium azide
Preservative 0.02% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 14055 Mouse EZH1

GeneID: 2145 Human EZH1

Swiss-port # P70351 Mouse Histone-lysine N-methyltransferase EZH1

Swiss-port # Q92800 Human Histone-lysine N-methyltransferase EZH1

Gene Symbol EZH1
Gene Full Name enhancer of zeste 1 polycomb repressive complex 2 subunit
Background EZH1 is a component of a noncanonical Polycomb repressive complex-2 (PRC2) that mediates methylation of histone H3 (see MIM 602812) lys27 (H3K27) and functions in the maintenance of embryonic stem cell pluripotency and plasticity (Shen et al., 2008 [PubMed 19026780]).[supplied by OMIM, Mar 2009]
Function Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH2-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. [UniProt]
Research Area Gene Regulation antibody
Calculated MW 85 kDa