ARG67066
anti-EZH2 antibody [SQab30336]
anti-EZH2 antibody [SQab30336] for IHC-Formalin-fixed paraffin-embedded sections and Human
Overview
| Product Description | Recombinant Rabbit Monoclonal antibody [SQab30336] recognizes EZH2 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SQab30336 |
| Isotype | IgG |
| Target Name | EZH2 |
| Antigen Species | Human |
| Immunogen | Recombinant protein fragment of EZH2 |
| Conjugation | Un-conjugated |
| Protein Full Name | EZH2 |
| Alternate Names | EZH2 ; Enhancer Of Zeste 2 Polycomb Repressive Complex 2 Subunit; ENX-1; KMT6 ; KMT6A; Histone-Lysine N-Methyltransferase EZH2; Lysine N-Methyltransferase 6; Enhancer Of Zeste Homolog 2; EZH1; Enhancer Of Zeste (Drosophila) Homolog 2; Enhancer Of Zeste Homolog 2 (Drosophila) 2 EC 2.1.1.43'); EC 2.1.1.356; EC 2.1.1.43; EC 2.1.1; EZH2b; ENX1; WVS2; WVS |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q15910 Human Histone-lysine N-methyltransferase EZH2 |
|---|---|
| Gene Symbol | EZH2 |
| Gene Full Name | enhancer of zeste 2 polycomb repressive complex 2 subunit |
| Background | This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Feb 2011] |
| Function | Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Displays a preference for substrates with less methylation, loses activity when progressively more methyl groups are incorporated into H3K27, H3K27me0 > H3K27me1 > H3K27me2. [UniProt] |
| Cellular Localization | Nucleus. [UniProt] |
| Calculated MW | 85 kDa |
| PTM | Glycoprotein, Isopeptide bond, Phosphoprotein,Ubl conjugation. [UniProt] |
