ARG62815

anti-EpCAM antibody [323/A3]

anti-EpCAM antibody [323/A3] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human

Controls and Markers antibody; Epithelial Marker antibody; Circulating Tumor Cells BioMarker antibody

Overview

Product Description Mouse Monoclonal antibody [323/A3] recognizes CD326 / EpCAM
Tested Reactivity Hu
Species Does Not React With Rat
Tested Application FACS, ICC/IF, IHC-P, IP, WB
Specificity The clone 323/A3 recognizes CD326 / EpCAM, a marker of epithelial lineages, that is over-expressed in many carcinomas.
Host Mouse
Clonality Monoclonal
Clone 323/A3
Isotype IgG1
Target Name EpCAM
Antigen Species Human
Immunogen Human breast cancer MCF-7 cells_x000D_
Conjugation Un-conjugated
Alternate Names MIC18; EGP; Tumor-associated calcium signal transducer 1; Epithelial glycoprotein 314; KSA; Ep-CAM; Epithelial cell surface antigen; Adenocarcinoma-associated antigen; HNPCC8; Cell surface glycoprotein Trop-1; EGP40; TACSTD1; KS1/4; hEGP314; Major gastrointestinal tumor-associated protein GA733-2; M4S1; MK-1; Epithelial glycoprotein; KS 1/4 antigen; ESA; DIAR5; EGP314; Epithelial cell adhesion molecule; EGP-2; TROP1; CD antigen CD326

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 4 µg/ml
ICC/IF1 - 10 µg/ml
IHC-P1 - 10 µg/ml
IP1 - 4 µg/ml / 100 - 500 µg of protein in 1 ml lysate
WB1 - 2 µg/ml
Application Note IHC-P: Pretreatment: Tissue section digestion with pepsin (15 min at RT or 10 min at 37°C, 1 mg / ml Tris-HCl, pH 2.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control WB: A431, MCF7 and SW480
IHC-P: Breast carcinoma

Properties

Form Liquid
Purification Purified from cell culture supernatant by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4072 Human EPCAM

Swiss-port # P16422 Human Epithelial cell adhesion molecule

Gene Symbol EPCAM
Gene Full Name epithelial cell adhesion molecule
Background EpCAM is a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]
Function EpCAM may act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E. [UniProt]
Research Area Controls and Markers antibody; Epithelial Marker antibody; Circulating Tumor Cells BioMarker antibody
Calculated MW 35 kDa
PTM Hyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. Glycosylation at Asn-198 is crucial for protein stability.

Clone References

The use of sensitive chemical antibodies for diagnosis: detection of low levels of EpCAM in breast cancer.

WB / Human

Shigdar S et al.
PLoS One.,  (2013)

publication_link

 

hr_line

Enrichment of circulating tumor cells from a large blood volume using leukapheresis and elutriation: proof of concept.

ICC/IF / Human

Eifler RL et al.
Cytometry B Clin Cytom.,  (2011)

publication_link

 

hr_line

Further evidence for EpCAM as the gene for congenital tufting enteropathy.

IHC-P / Human

Sivagnanam M et al.
Am J Med Genet A.,  (2010)

publication_link

 

hr_line

Overexpression of epithelial cell adhesion molecule in primary, metastatic, and recurrent/chemotherapy-resistant epithelial ovarian cancer: implications for epithelial cell adhesion molecule-specific immunotherapy.

IHC / Human

Bellone S et al.
Int J Gynecol Cancer.,  (2009)

publication_link

 

hr_line

Genetic vaccines against Ep-CAM break tolerance to self in a limited subset of subjects: initial identification of predictive biomarkers.

FACS / Mouse

Elia L et al.
Eur J Immunol.,  (2006)

publication_link

 

hr_line