ARG55250

anti-EpCAM antibody

anti-EpCAM antibody for Immunoprecipitation,Western blot and Human

Controls and Markers antibody; Epithelial Marker antibody; Circulating Tumor Cells BioMarker antibody
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Overview

Product Description

Mouse Monoclonal antibody recognizes EpCAM

Tested Reactivity Hu
Tested Application IP, WB
Specificity This antibody detects endogenous levels of EpCAM and does not cross-react with related proteins.
Host Mouse
Clonality Monoclonal
Isotype IgG1
Target Name EpCAM
Antigen Species Human
Immunogen Purified recombinant Human EpCAM protein fragments expressed in E.coli.
Conjugation Un-conjugated
Alternate Names MIC18; EGP; Tumor-associated calcium signal transducer 1; Epithelial glycoprotein 314; KSA; Ep-CAM; Epithelial cell surface antigen; Adenocarcinoma-associated antigen; HNPCC8; Cell surface glycoprotein Trop-1; EGP40; TACSTD1; KS1/4; hEGP314; Major gastrointestinal tumor-associated protein GA733-2; M4S1; MK-1; Epithelial glycoprotein; KS 1/4 antigen; ESA; DIAR5; EGP314; Epithelial cell adhesion molecule; EGP-2; TROP1; CD antigen CD326

Application Instructions

Application Suggestion
Tested Application Dilution
IPAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Purification Affinity purification with immunogen.
Buffer PBS (pH 7.4), 0.03% Proclin 300 and 50% Glycerol
Preservative 0.03% Proclin 300
Stabilizer 50% Glycerol
Concentration 6 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4072 Human EPCAM

Swiss-port # P16422 Human Epithelial cell adhesion molecule

Gene Symbol EPCAM
Gene Full Name epithelial cell adhesion molecule
Background EpCAM is a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]
Function EpCAM may act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E. [UniProt]
Cellular Localization Lateral cell membrane; Single-pass type I membrane protein. Cell junction > tight junction Note: Colocalizes with CLDN7 at the lateral cell membrane and tight junction.
Research Area Controls and Markers antibody; Epithelial Marker antibody; Circulating Tumor Cells BioMarker antibody
Calculated MW 35 kDa
PTM Hyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. Glycosylation at Asn-198 is crucial for protein stability.

Customer's Feedback

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Review for anti-EpCAM antibody

Application:WB

Sample:HCT116 and HT29

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC