ARG62816

anti-EpCAM antibody [VU-1D9] (FITC)

anti-EpCAM antibody [VU-1D9] (FITC) for Flow cytometry and Human

Controls and Markers antibody; Epithelial Marker antibody; Circulating Tumor Cells BioMarker antibody

Overview

Product Description FITC-conjugated Mouse Monoclonal antibody [VU-1D9] recognizes CD326 / EpCAM
Tested Reactivity Hu
Tested Application FACS
Specificity The clone VU-1D9 recognizes an epitope within EGF-like domain I of CD326 / EpCAM, a marker of epithelial lineages. This antibody strongly stains various normal epithelial cells and carcinomas.
Host Mouse
Clonality Monoclonal
Clone VU-1D9
Isotype IgG1
Target Name EpCAM
Immunogen Small cell lung carcinoma cell line H69.
Conjugation FITC
Alternate Names MIC18; EGP; Tumor-associated calcium signal transducer 1; Epithelial glycoprotein 314; KSA; Ep-CAM; Epithelial cell surface antigen; Adenocarcinoma-associated antigen; HNPCC8; Cell surface glycoprotein Trop-1; EGP40; TACSTD1; KS1/4; hEGP314; Major gastrointestinal tumor-associated protein GA733-2; M4S1; MK-1; Epithelial glycoprotein; KS 1/4 antigen; ESA; DIAR5; EGP314; Epithelial cell adhesion molecule; EGP-2; TROP1; CD antigen CD326

Application Instructions

Application Suggestion
Tested Application Dilution
FACS20 µl / 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Note The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4072 Human EPCAM

Swiss-port # P16422 Human Epithelial cell adhesion molecule

Gene Symbol EPCAM
Gene Full Name epithelial cell adhesion molecule
Background EpCAM is a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]
Function EpCAM may act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E. [UniProt]
Research Area Controls and Markers antibody; Epithelial Marker antibody; Circulating Tumor Cells BioMarker antibody
Calculated MW 35 kDa
PTM Hyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. Glycosylation at Asn-198 is crucial for protein stability.

Clone References

Superficial scrapings from breast tumors is a source for biobanking and research purposes.

Ma R et al.
Lab Invest.,  (2014)

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Multiple breast cancer cell-lines derived from a single tumor differ in their molecular characteristics and tumorigenic potential.

FACS / Human

Mosoyan G et al.
PLoS One.,  (2013)

publication_link

 

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Differential gene expression in normal esophagus and Barrett's esophagus.

IHC / Human

Wang J et al.
J Gastroenterol.,  (2009)

publication_link

 

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Heritable somatic methylation and inactivation of MSH2 in families with Lynch syndrome due to deletion of the 3' exons of TACSTD1.

IHC / Human

Ligtenberg MJ et al.
Nat Genet.,  (2009)

publication_link

 

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