ARG58618

anti-Ephrin A1 antibody

anti-Ephrin A1 antibody for Western blot and Mouse,Rat

Overview

Product Description Goat Polyclonal antibody recognizes Ephrin A1
Tested Reactivity Ms, Rat
Predict Reactivity Hu, Dog, Pig
Tested Application WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name Ephrin A1
Antigen Species Human
Immunogen Synthetic peptide from the internal region of Human Ephrin A1 (NP_004419.2). (C-KVTVSGKITHSP)
Conjugation Un-conjugated
Alternate Names ECKLG; B61; EPLG1; TNFAIP4; Immediate early response protein B61; Tumor necrosis factor alpha-induced protein 4; EFL1; LERK-1; EPH-related receptor tyrosine kinase ligand 1; LERK1; TNF alpha-induced protein 4; Ephrin-A1

Application Instructions

Application Suggestion
Tested Application Dilution
WB0.01 - 0.03 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~ 26 kDa

Properties

Form Liquid
Purification Ammonium sulphate precipitation followed by affinity purification with immunogen.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 13636 Mouse EFNA1

GeneID: 94268 Rat EFNA1

Swiss-port # P52793 Mouse Ephrin-A1

Swiss-port # P97553 Rat Ephrin-A1

Gene Symbol EFNA1
Gene Full Name ephrin-A1
Background This gene encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNA class ephrin which binds to the EPHA2, EPHA4, EPHA5, EPHA6, and EPHA7 receptors. Two transcript variants that encode different isoforms were identified through sequence analysis. [provided by RefSeq, Jul 2008]
Function Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. Plays an important role in angiogenesis and tumor neovascularization. The recruitment of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly. Exerts anti-oncogenic effects in tumor cells through activation and down-regulation of EPHA2. Activates EPHA2 by inducing tyrosine phosphorylation which leads to its internalization and degradation. Acts as a negative regulator in the tumorigenesis of gliomas by down-regulating EPHA2 and FAK. Can evoke collapse of embryonic neuronal growth cone and regulates dendritic spine morphogenesis. [UniProt]
Calculated MW 24 kDa
PTM Undergoes proteolysis by a metalloprotease to give rise to a soluble monomeric form.

N-Glycosylation is required for binding to EPHA2 receptor and inducing its internalization. [UniProt]