ARG51684
anti-Ephrin B2 phospho (Tyr330) antibody
anti-Ephrin B2 phospho (Tyr330) antibody for Western blot and Human,Mouse
Cancer antibody; Cell Biology and Cellular Response antibody; Neuroscience antibody
Overview
Product Description | Rabbit Polyclonal antibody recognizes Ephrin B2 phospho (Tyr330) |
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Tested Reactivity | Hu, Ms |
Tested Application | WB |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Target Name | Ephrin B2 |
Antigen Species | Human |
Immunogen | Peptide sequence around phosphorylation site of tyrosine 330 (N-I-Y(p)-Y-K) derived from Human Ephrin-B2 |
Conjugation | Un-conjugated |
Alternate Names | HTK ligand; HTKL; Htk-L; EPLG5; Ephrin-B2; HTK-L; LERK5; LERK-5; EPH-related receptor tyrosine kinase ligand 5 |
Application Instructions
Application Suggestion |
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Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
Form | Liquid |
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Purification | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic peptide. Antibodies were purified by affinity-chromatography using epitope-specific peptide. |
Buffer | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
Preservative | 0.02% Sodium azide |
Stabilizer | 50% Glycerol |
Concentration | 1 mg/ml |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links | |
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Gene Symbol | EFNB2 |
Gene Full Name | ephrin-B2 |
Background | Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described. |
Function | Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to receptor tyrosine kinase including EPHA4, EPHA3 and EPHB4. Together with EPHB4 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. May play a role in constraining the orientation of longitudinally projecting axons. [UniProt] |
Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Neuroscience antibody |
Calculated MW | 37 kDa |
PTM | Inducible phosphorylation of tyrosine residues in the cytoplasmic domain. |