ARG57444
anti-Ezrin antibody
anti-Ezrin antibody for Western blot and Human,Mouse,Rat
Overview
| Product Description | Mouse Monoclonal antibody recognizes Ezrin |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | WB |
| Specificity | This antibody detects endogenous levels of Ezrin and does not cross-react with related proteins. |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Target Name | Ezrin |
| Antigen Species | Human |
| Immunogen | Purified recombinant Human Ezrin protein fragments expressed in E. coli. |
| Conjugation | Un-conjugated |
| Alternate Names | Ezrin; p81; HEL-S-105; VIL2; CVL; Cytovillin; CVIL; Villin-2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.4), 0.03% Proclin300 and 50% Glycerol. |
| Preservative | 0.03% Proclin300 |
| Stabilizer | 50% Glycerol |
| Concentration | 3.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | EZR |
| Gene Full Name | ezrin |
| Background | The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene. [provided by RefSeq, Jul 2008] |
| Function | Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis. [UniProt] |
| Cellular Localization | Apical cell membrane. Cell projection. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Localization to the apical membrane of parietal cells depends on the interaction with MPP5. Localizes to cell extensions and peripheral processes of astrocytes (By similarity). Microvillar peripheral membrane protein. |
| Calculated MW | 69 kDa |
| PTM | Phosphorylated by tyrosine-protein kinases. Phosphorylation by ROCK2 suppresses the head-to-tail association of the N-terminal and C-terminal halves resulting in an opened conformation which is capable of actin and membrane-binding (By similarity). S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. |
