ARG23737

anti-F-actin antibody [NH3]

anti-F-actin antibody [NH3] for ELISA,Flow cytometry,ICC/IF,IHC-Frozen sections,Immunoprecipitation,Western blot and Human,Mouse,Rabbit,Rat

Controls and Markers antibody; Signaling Transduction antibody; Loading Control antibody; Cytochrome-C fractionation Study antibody; Inflammation Study antibody; Tag Internal Control antibody

Overview

Product Description Mouse Monoclonal antibody [NH3] recognizes F-actin.
Tested Reactivity Hu, Ms, Rat, Rb
Tested Application ELISA, FACS, ICC/IF, IHC-Fr, IP, WB
Specificity The clone [NH3] recognises human Filamentous actin (F-actin) and reportedly also recognizes Globular actin (G-actin). The antibody binds to the N-terminal region of actin, but not to the extreme N-terminal 40 amino acids. In tissue sections the antibody stains the cytoplasm of macrophages strongly, and gives granular, localised nuclear staining of all cell types. As this is a mouse IgM antibody, an anti-mouse IgM secondary antibody is recommended to use to detect this primary antibody.
Host Mouse
Clonality Monoclonal
Clone NH3
Isotype IgM
Target Name F-actin
Antigen Species Human
Immunogen Human monocytes and U937 cell line.
Conjugation Un-conjugated
Alternate Names PS1TP5BP1; BRWS1; Actin, cytoplasmic 1; Beta-actin

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA1:10
FACS1:10
ICC/IF1:100
IHC-FrAssay-dependent
IPAssay-dependent
WB1:100 - 1:500
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified.
Buffer PBS and 0.09% Sodium azide.
Preservative 0.09% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 100009272 Rabbit ACTB

GeneID: 11461 Mouse ACTB

GeneID: 60 Human ACTB

Gene Symbol ACTB
Background This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008]
Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. [UniProt]
Research Area Controls and Markers antibody; Signaling Transduction antibody; Loading Control antibody; Cytochrome-C fractionation Study antibody; Inflammation Study antibody; Tag Internal Control antibody
Calculated MW 42 kDa