ARG43814
anti-F2RL1 antibody [22F95]
anti-F2RL1 antibody [22F95] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit monoclonal antibody [22F95] recognizes F2RL1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | 22F95 |
| Isotype | IgG |
| Target Name | F2RL1 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide of Human F2RL1. |
| Conjugation | Un-conjugated |
| Protein Full Name | Proteinase-activated receptor 2 |
| Alternate Names | F2RL1, F2R Like Trypsin Receptor 1 , PAR2, GPR11, Coagulation Factor II (Thrombin) Receptor-Like 1, Proteinase-Activated Receptor 2, G-Protein Coupled Receptor 11, Thrombin Receptor-Like 1, Coagulation Factor II Receptor-Like 1, Proteinase-Activated Receptor-2 , Protease-Activated Receptor 2, PAR-2 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|---|---|
| Positive Control | K562 | ||||||||
| Observed Size | 52~60 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity chromatography purification |
| Buffer | PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide, 50% Glycerol and 0.4 - 0.5 mg/ml BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol and 0.4 - 0.5 mg/ml BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | F2RL1 |
| Gene Full Name | F2R Like Trypsin Receptor 1 |
| Background | This gene encodes a member of the G-protein coupled receptor 1 family of proteins. The encoded cell surface receptor is activated through proteolytic cleavage of its extracellular amino terminus, resulting in a new amino terminus that acts as a tethered ligand that binds to an extracellular loop domain. Activation of the receptor has been shown to stimulate vascular smooth muscle relaxation, dilate blood vessels, increase blood flow, and lower blood pressure. This protein is also important in the inflammatory response, as well as innate and adaptive immunity. [provided by RefSeq, Jun 2016] |
| Function | Can also be transactivated by cleaved F2R/PAR1. Involved in modulation of inflammatory responses and regulation of innate and adaptive immunity, and acts as a sensor for proteolytic enzymes generated during infection. Generally is promoting inflammation. Can signal synergistically with TLR4 and probably TLR2 in inflammatory responses and modulates TLR3 signaling. Has a protective role in establishing the endothelial barrier; the activity involves coagulation factor X. Regulates endothelial cell barrier integrity during neutrophil extravasation, probably following proteolytic cleavage by PRTN3. [UniProt] |
| Cellular Localization | Cell membrane; Membrane |
| Calculated MW | 44 kDa |
| PTM | N-glycosylated and sialylated.1 Multiple phosphorylated on serine and threonine residues in the cytoplasmic region upon receptor activation; required for receptor desensitization and recruitment of beta-arrestin.1 Monoubiquitinated by CBL at the plasma membrane and in early endosomes; not required for receptor endocytosis but for translocation to late endosomes or lysosomes. Deubiquitination involves STAMBP and USP8; required for lysosomal trafficking and receptor degradation. [UniProt] |
