ARG64242

anti-FACT / SUPT16H antibody

anti-FACT / SUPT16H antibody for Western blot and Human

Cell Biology and Cellular Response antibody; Gene Regulation antibody; Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes FACT / SUPT16H
Tested Reactivity Hu
Predict Reactivity Ms, Cow, Pig
Tested Application WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name FACT / SUPT16H
Antigen Species Human
Immunogen C-DLTNKEGKKPEEK
Conjugation Un-conjugated
Alternate Names Chromatin-specific transcription elongation factor 140 kDa subunit; FACTP140; FACT complex subunit SPT16; SPT16; FACTp140; CDC68; FACT 140 kDa subunit; Facilitates chromatin transcription complex subunit SPT16; SPT16/CDC68; hSPT16

Application Instructions

Application Suggestion
Tested Application Dilution
WB0.1 - 0.3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 11198 Human SUPT16H

Swiss-port # Q9Y5B9 Human FACT complex subunit SPT16

Background Transcription of protein-coding genes can be reconstituted on naked DNA with only the general transcription factors and RNA polymerase II. However, this minimal system cannot transcribe DNA packaged into chromatin, indicating that accessory factors may facilitate access to DNA. One such factor, FACT (facilitates chromatin transcription), interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT is composed of an 80 kDa subunit and a 140 kDa subunit; this gene encodes the 140 kDa subunit. [provided by RefSeq, Feb 2009]
Research Area Cell Biology and Cellular Response antibody; Gene Regulation antibody; Signaling Transduction antibody
Calculated MW 120 kDa
PTM ADP-ribosylated. ADP-ribosylation by PARP1 is induced by genotoxic stress and correlates with dissociation of FACT from chromatin.