ARG54127

anti-FADD antibody

anti-FADD antibody for Immunoprecipitation,Western blot and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody
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Overview

Product Description

Mouse Monoclonal antibody recognizes FADD

Tested Reactivity Hu
Tested Application IP, WB
Host Mouse
Clonality Monoclonal
Isotype IgG1
Target Name FADD
Antigen Species Human
Immunogen Purified recombinant human FADD protein fragments expressed in E.coli.
Conjugation Un-conjugated
Alternate Names Mediator of receptor induced toxicity; MORT1; GIG3; FAS-associated death domain protein; Growth-inhibiting gene 3 protein; Protein FADD; FAS-associating death domain-containing protein

Application Instructions

Application Suggestion
Tested Application Dilution
IPAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purified
Buffer 0.1M Tris-Glycine (pH 7.4), 150 mM NaCl, 0.2% Sodium azide and 50% Glycerol
Preservative 0.2% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 8772 Human FADD

Swiss-port # Q13158 Human FAS-associated death domain protein

Gene Symbol FADD
Gene Full Name Fas (TNFRSF6)-associated via death domain
Background Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors.The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation.Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis.Involved in interferon-mediated antiviral immune response,playing a role in the positive regulation of interferon signaling.
Function Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis. Involved in interferon-mediated antiviral immune response, playing a role in the positive regulation of interferon signaling. [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody
Calculated MW 23 kDa

Customer's Feedback

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Review for anti-FADD antibody

Application:WB

Sample:293T and HeLa

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC