ARG56961

anti-FADD antibody [J1D2]

anti-FADD antibody [J1D2] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Overview

Product Description Mouse Monoclonal antibody [J1D2] recognizes FADD
Tested Reactivity Hu
Tested Application IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone J1D2
Isotype IgG2b, kappa
Target Name FADD
Antigen Species Human
Immunogen Recombinant fragment around aa. 1-208 of Human FADD.
Conjugation Un-conjugated
Alternate Names Mediator of receptor induced toxicity; MORT1; GIG3; FAS-associated death domain protein; Growth-inhibiting gene 3 protein; Protein FADD; FAS-associating death domain-containing protein

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50
WB1:1000 - 1:2000
Application Note IHC-P: Antigen Retrieval: Boil tissue section in 0.1M Sodium citrate buffer (pH 6.0) for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 10% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 8772 Human FADD

Swiss-port # Q13158 Human FAS-associated death domain protein

Gene Symbol FADD
Gene Full Name Fas (TNFRSF6)-associated via death domain
Background The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development. [provided by RefSeq, Jul 2008]
Function Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis. Involved in interferon-mediated antiviral immune response, playing a role in the positive regulation of interferon signaling. [UniProt]
Calculated MW 23 kDa