ARG43430

anti-FAM120A antibody

anti-FAM120A antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human

Overview

Product Description Mouse Monoclonal antibody recognizes FAM120A
Tested Reactivity Hu
Tested Application IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone 1501CT803.19.85.5
Isotype IgG1, kappa
Target Name FAM120A
Antigen Species Human
Immunogen Recombinant protein of Human FAM120A.
Conjugation Un-conjugated
Alternate Names Constitutive coactivator of PPAR-gamma-like protein 1; Protein FAM120A; C9orf10; Oxidative stress-associated Src activator; DNAPTP5; DNAPTP1; OSSA

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:25
WB1:4000
Application Note IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control MCF7
Observed Size ~ 135 kDa

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS and 0.09% (W/V) Sodium azide.
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 23196 Human FAM120A

Swiss-port # Q9NZB2 Human Constitutive coactivator of PPAR-gamma-like protein 1

Gene Symbol FAM120A
Gene Full Name family with sequence similarity 120A
Function May participate in mRNA transport in the cytoplasm (By similarity). Critical component of the oxidative stress-induced survival signaling. Activates src family kinases and acts as a scaffolding protein enabling src family kinases to phosphorylate and activate PI3-kinase. Binds RNA and promotes the secretion of IGF-II. May play a pivotal role in the progression of scirrhous-type gastric cancer by supporting cancer cell survival in environments with various oxidative stresses. [UniProt]
Cellular Localization Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note=Translocates to plasma membrane upon ultraviolet exposure. [UniProt]
Calculated MW 122 kDa
PTM Arg-982 is dimethylated, probably to asymmetric dimethylarginine.

Phosphorylated on tyrosine by src family kinases upon ultraviolet exposure. [UniProt]