ARG52279

anti-FANCI phospho (Ser556) antibody

anti-FANCI phospho (Ser556) antibody for Western blot and Human

Gene Regulation antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes FANCI phospho (Ser556)
Tested Reactivity Hu
Predict Reactivity Bov, Goat, NHuPrm, Sheep
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name FANCI
Antigen Species Human
Immunogen Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser556 conjugated to KLH
Conjugation Un-conjugated
Alternate Names Fanconi anemia group I protein; KIAA1794; Protein FACI

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:1,000
Application Note Specific for the ~150k FANCI protein phosphorylated at Ser556 . Immunolabeling of FANCI is completely eliminated by lambda-phosphatase treatment.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity Purified
Buffer 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol
Stabilizer 0.1 mg/ml BSA, 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 55215 Human FANCI

Swiss-port # Q9NVI1 Human Fanconi anemia group I protein

Gene Symbol FANCI
Gene Full Name Fanconi anemia, complementation group I
Background Fanconi anemia, FA, is a rare disorder where cells cannot prevent, repair, or tolerate DNA damage, leading to cancer, progressive bone marrow failure and developmental abnormalities (Ishiai et al, 2008). 16 genes have been implicated in FA, and their products constitute a common FA pathway (Walden and Deans 2014). FANCI (Fanconi anemia complementation group I), is one of two substrates for monoubiquitnation by the FANCL-containing core complex, and is crucial for DNA repair via FAP and intrastrand cross-links (Walden and Deans, 2014). Phosphorylation at Ser 556 has been shown to play a key role in FANCI physically associating with FANCD2, stabilizing the ID2 complex, and supporting its DNA binding and subsequent D2-ubiquitination (Walden and Deans, 2014).
Research Area Gene Regulation antibody
Calculated MW 149 kDa
PTM Monoubiquitinated by FANCL on Lys-523 during S phase and upon genotoxic stress. Deubiquitinated by USP1 as cells enter G2/M, or once DNA repair is completed. Monoubiquitination requires the FANCA-FANCB-FANCC-FANCE-FANCF-FANCG-FANCM complex. Ubiquitination is required for binding to chromatin, DNA repair, and normal cell cycle progression. Monoubiquitination is stimulated by DNA-binding.
Phosphorylated in response to DNA damage by ATM and/or ATR.