ARG56646

anti-FGF basic antibody

anti-FGF basic antibody for ELISA,Western blot,Neutralizing and Human,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes FGF basic
Tested Reactivity Hu, Rat
Tested Application ELISA, Neut, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name FGF basic
Antigen Species Human
Immunogen E.coli derived Recombinant Human FGF basic (154 aa).
(AAGSITTLPA LPEDGGSGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHIK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTDEC FFFERLESNN YNTYRSRKYT SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS)
Conjugation Un-conjugated
Alternate Names FGF-2; Fibroblast growth factor 2; bFGF; FGFB; Heparin-binding growth factor 2; BFGF; HBGF-2; Basic fibroblast growth factor

Application Instructions

Application Suggestion
Tested Application Dilution
ELISASandwich: 0.5 - 2.0 µg/ml with ARG56756 as a detection antibody
Neut0.25 - 0.40 µg/ml (To yield [ND50] of the biological activity of Human FGF - basic (0.3 ng/ml) )
WB0.1 - 0.2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2247 Human FGF2

GeneID: 54250 Rat FGF2

Swiss-port # P09038 Human Fibroblast growth factor 2

Swiss-port # P13109 Rat Fibroblast growth factor 2

Gene Symbol FGF2
Gene Full Name fibroblast growth factor 2 (basic)
Background The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. [provided by RefSeq, Jul 2008]
Function Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. [UniProt]
Highlight Related products:
FGF basic antibodies; FGF basic ELISA Kits; FGF basic recombinant proteins; Anti-Rabbit IgG secondary antibodies;
Related news:
The role of HDGF in tumor angiogenesis
Calculated MW 31 kDa
PTM Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.
Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing.