ARG64251

anti-FH / Fumarase antibody

anti-FH / Fumarase antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Cancer antibody; Metabolism antibody; Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes FH / Fumarase
Tested Reactivity Hu
Predict Reactivity Ms, Rat, Dog
Tested Application IHC-P, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name FH / Fumarase
Antigen Species Human
Immunogen C-HPNDHVNKSQSSND
Conjugation Un-conjugated
Alternate Names MCUL1; MCL; LRCC; EC 4.2.1.2; Fumarate hydratase, mitochondrial; FMRD; Fumarase; HLRCC

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P3 - 5 µg/ml
WB0.01 - 0.03 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2271 Human FH

Swiss-port # P07954 Human Fumarate hydratase, mitochondrial

Background The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy. [provided by RefSeq, Jul 2008]
Highlight Related products:
Fumarase antibodies; Fumarase ELISA Kits; Anti-Goat IgG secondary antibodies;
Related news:
TCA intermediate fumarate promotes mitobiogenesis
Research Area Cancer antibody; Metabolism antibody; Signaling Transduction antibody
Calculated MW 55 kDa