ARG63657

anti-FHL1 / SLIM1 antibody

anti-FHL1 / SLIM1 antibody for Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat

Cell Biology and Cellular Response antibody; Gene Regulation antibody

Overview

Product Description Goat Polyclonal antibody recognizes FHL1 / SLIM1
Tested Reactivity Hu, Ms, Rat
Predict Reactivity Cow, Dog, Pig
Tested Application FACS, ICC/IF, WB
Specificity This antibody is expected to recognise isoforms 2( NP_001440.1) and 5 (NP_001153171.1). Since June 2009 the second N residue in the immunizing peptide is no longer representative as it has been substituted by Q at position 284 of NP_001440.2. Variants NP_001440.2, NP_001153176.1 and NP_001153172.1 represent identical protein.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name FHL1 / SLIM1
Antigen Species Human
Immunogen CNKRFVFHNEQVY
Conjugation Un-conjugated
Alternate Names FHL1A; FHL1B; KYOT; Four and a half LIM domains protein 1; SLIM; FLH1A; XMPMA; FHL-1; SLIM-1; SLIM1; SLIMMER; Skeletal muscle LIM-protein 1

Application Instructions

Application Suggestion
Tested Application Dilution
FACS10 µg/ml
ICC/IF10 µg/ml
WB0.05 - 0.3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by antigen affinity chromatography.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 14199 Mouse FHL1

GeneID: 2273 Human FHL1

GeneID: 25177 Rat FHL1

Background This gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. Expression of these family members occurs in a cell- and tissue-specific mode and these proteins are involved in many cellular processes. Mutations in this gene have been found in patients with Emery-Dreifuss muscular dystrophy. Multiple alternately spliced transcript variants which encode different protein isoforms have been described.[provided by RefSeq, Nov 2009]
Research Area Cell Biology and Cellular Response antibody; Gene Regulation antibody
Calculated MW 36 kDa