ARG40692

anti-FLIP antibody

anti-FLIP antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes FLIP
Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name FLIP
Antigen Species Human
Immunogen Recombinant protein corresponding to M1-D376 of Human FLIP.
Conjugation Un-conjugated
Alternate Names FLAME1; CASP8AP1; c-FLIPL; FLIP; c-FLIPR; c-FLIPS; MACH-related inducer of toxicity; Inhibitor of FLICE; c-FLIP; CASH; Cellular FLICE-like inhibitory protein; CASP8 and FADD-like apoptosis regulator; Caspase homolog; FLAME; FADD-like antiapoptotic molecule 1; Caspase-eight-related protein; Caspase-like apoptosis regulatory protein; I-FLICE; FLAME-1; MRIT; Casper; Usurpin; CLARP

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:200 - 1:1000
WB1:500 - 1:2000
Application Note IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose.
Preservative 0.05% Sodium azide
Stabilizer 4% Trehalose
Concentration 0.5 - 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12633 Mouse CFLAR

GeneID: 8837 Human CFLAR

Swiss-port # O15519 Human CASP8 and FADD-like apoptosis regulator

Swiss-port # O35732 Mouse CASP8 and FADD-like apoptosis regulator

Gene Symbol CFLAR
Gene Full Name CASP8 and FADD-like apoptosis regulator
Background The protein encoded by this gene is a regulator of apoptosis and is structurally similar to caspase-8. However, the encoded protein lacks caspase activity and appears to be itself cleaved into two peptides by caspase-8. Several transcript variants encoding different isoforms have been found for this gene, and partial evidence for several more variants exists. [provided by RefSeq, Feb 2011]
Function Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity. [UniProt]
Calculated MW 55 kDa
PTM Proteolytically processed; probably by caspase-8. Processing likely occurs at the DISC and generates subunit p43 and p12. [UniProt]