ARG54328

anti-FLIP antibody

anti-FLIP antibody for ICC/IF,Western blot and Human,Mouse,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody
publication_link Publication1

Overview

Product Description Rabbit Polyclonal antibody recognizes FLIP
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, WB
Specificity This antibody recognizes all human, mouse, and rat FLIP splice variants.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name FLIP
Antigen Species Human
Immunogen Peptide corresponding to aa 2-18 of human FLIP (accession no. AAC51622). This sequence is identical in all FLIP splice variants.
Conjugation Un-conjugated
Alternate Names FLAME1; CASP8AP1; c-FLIPL; FLIP; c-FLIPR; c-FLIPS; MACH-related inducer of toxicity; Inhibitor of FLICE; c-FLIP; CASH; Cellular FLICE-like inhibitory protein; CASP8 and FADD-like apoptosis regulator; Caspase homolog; FLAME; FADD-like antiapoptotic molecule 1; Caspase-eight-related protein; Caspase-like apoptosis regulatory protein; I-FLICE; FLAME-1; MRIT; Casper; Usurpin; CLARP

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IFAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HeLa and K562

Properties

Form Liquid
Purification Immunoaffinity chroma-tography
Buffer PBS (pH 7.4) and 0.02% Sodium azide
Preservative 0.02% Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12633 Mouse CFLAR

GeneID: 8837 Human CFLAR

Swiss-port # O15519 Human CASP8 and FADD-like apoptosis regulator

Swiss-port # O35732 Mouse CASP8 and FADD-like apoptosis regulator

Gene Symbol CFLAR
Gene Full Name CASP8 and FADD-like apoptosis regulator
Background Caspase-8 (FLICE) and -10 (FLICE2) are two pivotal members of the ICE/CED-3 protease family. FLICE-inhibitory proteins have been identified in viruses and human cells and are designated v-FLIPs and FLIP, respectively. Human FLIP was cloned by several independent laboratories and designated Casper, I-FLICE, FLAME-1, CASH, and CLARP. FLIP contains two death effector domains and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and -10 and potently inhibits apoptosis induced by all known death receptors.
Function Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity. [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody
Calculated MW 55 kDa
PTM Proteolytically processed; probably by caspase-8. Processing likely occurs at the DISC and generates subunit p43 and p12.

Specific References

Wentilactone A induces cell apoptosis by targeting AKR1C1 gene via the IGF‑1R/IRS1/PI3K/AKT/Nrf2/FLIP/Caspase‑3 signaling pathway in small cell lung cancer.

WB / Human

Jiang Wenli et al.
Oncology Letters.,  (2018)

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