ARG54399
anti-FLIP gamma + delta antibody
anti-FLIP gamma + delta antibody for ICC/IF,Western blot and Human,Mouse
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes FLIP gamma + delta |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ICC/IF, WB |
| Specificity | This antibody recognizes human FLIP gamma (35kDa) and FLIP delta (25kDa) but not FLIP alpha or FLIP beta. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | FLIP gamma + delta |
| Antigen Species | Human |
| Immunogen | Peptide corresponding to aa 191-209 at the C-terminus of human FLIP /FLIP S form (accession no. AAC51623). |
| Conjugation | Un-conjugated |
| Alternate Names | FLAME1; CASP8AP1; c-FLIPL; FLIP; c-FLIPR; c-FLIPS; MACH-related inducer of toxicity; Inhibitor of FLICE; c-FLIP; CASH; Cellular FLICE-like inhibitory protein; CASP8 and FADD-like apoptosis regulator; Caspase homolog; FLAME; FADD-like antiapoptotic molecule 1; Caspase-eight-related protein; Caspase-like apoptosis regulatory protein; I-FLICE; FLAME-1; MRIT; Casper; Usurpin; CLARP |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | HeLa, Jurkat, THP-1, A431, K562 and NIH/3T3 |
Properties
| Form | Liquid |
|---|---|
| Purification | Immunoaffinity chroma-tography |
| Buffer | PBS (pH 7.4) and 0.02% Sodium azide |
| Preservative | 0.02% Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O15519 Human CASP8 and FADD-like apoptosis regulator Swiss-port # O35732 Mouse CASP8 and FADD-like apoptosis regulator |
|---|---|
| Gene Symbol | CFLAR |
| Gene Full Name | CASP8 and FADD-like apoptosis regulator |
| Background | Caspase-8 (FLICE) and -10 (FLICE2) are twopivotal members of the ICE/CED-3 proteasefamily. FLICE-inhibitory proteins have beenidentified in viruses and human cells and aredesignated v-FLIPs and FLIP, respectively.Human FLIP was cloned by several independentlaboratories and designated Casper, I-FLICE,FLAME-1, CASH, and CLARP. FLIP containstwo death effector domains and a caspase-likedomain. FLIP interacts with adapter proteinFADD and caspase-8 and -10 and potentlyinhibits apoptosis induced by death receptorsCD95, DR3, TRAIL-R, and TNFR1. Four splicevariants of FLIP have been identified anddesignated FLIP alpha, beta, gamma, and delta. |
| Function | Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity. [UniProt] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | FLIP gamma (35 kDa) and FLIP delta (25 kDa) |
| PTM | Proteolytically processed; probably by caspase-8. Processing likely occurs at the DISC and generates subunit p43 and p12. |
