ARG54399

anti-FLIP gamma + delta antibody

anti-FLIP gamma + delta antibody for ICC/IF,Western blot and Human,Mouse

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes FLIP gamma + delta
Tested Reactivity Hu, Ms
Tested Application ICC/IF, WB
Specificity This antibody recognizes human FLIP gamma (35kDa) and FLIP delta (25kDa) but not FLIP alpha or FLIP beta.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name FLIP gamma + delta
Antigen Species Human
Immunogen Peptide corresponding to aa 191-209 at the C-terminus of human FLIP /FLIP S form (accession no. AAC51623).
Conjugation Un-conjugated
Alternate Names FLAME1; CASP8AP1; c-FLIPL; FLIP; c-FLIPR; c-FLIPS; MACH-related inducer of toxicity; Inhibitor of FLICE; c-FLIP; CASH; Cellular FLICE-like inhibitory protein; CASP8 and FADD-like apoptosis regulator; Caspase homolog; FLAME; FADD-like antiapoptotic molecule 1; Caspase-eight-related protein; Caspase-like apoptosis regulatory protein; I-FLICE; FLAME-1; MRIT; Casper; Usurpin; CLARP

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IFAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HeLa, Jurkat, THP-1, A431, K562 and NIH/3T3

Properties

Form Liquid
Purification Immunoaffinity chroma-tography
Buffer PBS (pH 7.4) and 0.02% Sodium azide
Preservative 0.02% Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12633 Mouse CFLAR

GeneID: 8837 Human CFLAR

Swiss-port # O15519 Human CASP8 and FADD-like apoptosis regulator

Swiss-port # O35732 Mouse CASP8 and FADD-like apoptosis regulator

Gene Symbol CFLAR
Gene Full Name CASP8 and FADD-like apoptosis regulator
Background Caspase-8 (FLICE) and -10 (FLICE2) are twopivotal members of the ICE/CED-3 proteasefamily. FLICE-inhibitory proteins have beenidentified in viruses and human cells and aredesignated v-FLIPs and FLIP, respectively.Human FLIP was cloned by several independentlaboratories and designated Casper, I-FLICE,FLAME-1, CASH, and CLARP. FLIP containstwo death effector domains and a caspase-likedomain. FLIP interacts with adapter proteinFADD and caspase-8 and -10 and potentlyinhibits apoptosis induced by death receptorsCD95, DR3, TRAIL-R, and TNFR1. Four splicevariants of FLIP have been identified anddesignated FLIP alpha, beta, gamma, and delta.
Function Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity. [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody
Calculated MW FLIP gamma (35 kDa) and FLIP delta (25 kDa)
PTM Proteolytically processed; probably by caspase-8. Processing likely occurs at the DISC and generates subunit p43 and p12.