ARG10712

anti-FOX3 / NeuN antibody

anti-FOX3 / NeuN antibody for ICC/IF,IHC-Frozen sections,Western blot and Cow,Horse,Human,Mouse,Pig,Rat

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Overview

Product Description

Rabbit Polyclonal antibody recognizes FOX3 / NeuN

Tested Reactivity Hu, Ms, Rat, Cow, Hrs, Pig
Predict Reactivity Chk
Tested Application ICC/IF, IHC-Fr, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name FOX3 / NeuN
Antigen Species Human
Immunogen N-terminal 100 aa. of Human Fox3 expressed in and purified from E. coli.
Conjugation Un-conjugated
Alternate Names RNA binding protein fox-1 homolog 3; NEUN; FOX-3; HRNBP3; Fox-1 homolog C; FOX3

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:500 - 1:1000
IHC-Fr1:500 - 1:1000
WB1:5000 - 1:10000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification.
Buffer PBS and 50% Glycerol.
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 146713 Human RBFOX3

GeneID: 52897 Mouse RBFOX3

Swiss-port # A6NFN3 Human RNA binding protein fox-1 homolog 3

Swiss-port # Q8BIF2 Mouse RNA binding protein fox-1 homolog 3

Gene Symbol RBFOX3
Gene Full Name RNA binding protein, fox-1 homolog (C. elegans) 3
Function RNA-binding protein that regulates alternative splicing events. [UniProt]
Calculated MW 34 kDa

Customer's Feedback

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Review for anti-FOX3 / NeuN antibody

Application:IHC

Sample:Mouse C57BL/6Jnarl brain tissue.

Sample Preparation Method:Frozen

Fixation Buffer:4% formalin

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Conjugation of Secondary Antibody:Dylight 594

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Review for anti-FOX3 / NeuN antibody

Application:WB

Sample:U87, Mouse brain and Rat brain

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:5000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

Comparison of Different Tissue Clearing Methods for Three-Dimensional Reconstruction of Human Brain Cellular Anatomy Using Advanced Imaging Techniques

IHC-Fr / Human

Marina Scardigli et al.
Front Neuroanat.,  (2021)

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