ARG57595
anti-FOXM1 antibody
anti-FOXM1 antibody for ChIP,ICC/IF,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes FOXM1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ChIP, ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | FOXM1 |
| Antigen Species | Human |
| Immunogen | Recombinant protein of Human FOXM1. |
| Conjugation | Un-conjugated |
| Alternate Names | MPP-2; TRIDENT; MPHOSPH2; Forkhead-related protein FKHL16; HFH11; HNF-3/fork-head homolog 11; MPP2; FKHL16; HFH-11; Transcription factor Trident; INS-1; M-phase phosphoprotein 2; HNF-3; FOXM1B; Hepatocyte nuclear factor 3 forkhead homolog 11; Winged-helix factor from INS-1 cells; Forkhead box protein M1; PIG29; MPM-2 reactive phosphoprotein 2 |
Application Instructions
| Application Suggestion |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | HeLa | ||||||||
| Observed Size | 108 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | FOXM1 |
| Gene Full Name | forkhead box M1 |
| Background | The protein encoded by this gene is a transcriptional activator involved in cell proliferation. The encoded protein is phosphorylated in M phase and regulates the expression of several cell cycle genes, such as cyclin B1 and cyclin D1. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011] |
| Function | Transcriptional factor regulating the expression of cell cycle genes essential for DNA replication and mitosis. Plays a role in the control of cell proliferation. Plays also a role in DNA breaks repair participating in the DNA damage checkpoint response. [UniProt] |
| Calculated MW | 84 kDa |
| PTM | Phosphorylated in M (mitotic) phase. Phosphorylation by the checkpoint kinase CHEK2 in response to DNA damage increases the FOXM1 protein stability probably stimulating the transcription of genes involved in DNA repair. Phosphorylated by CDK1 in late S and G2 phases, creating docking sites for the POLO box domains of PLK1. Subsequently, PLK1 binds and phosphorylates FOXM1, leading to activation of transcriptional activity and subsequent enhanced expression of key mitotic regulators. [UniProt] |
