ARG64738

anti-FTO antibody

anti-FTO antibody for Western blot and Human

Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody

Overview

Product Description Goat Polyclonal antibody recognizes FTO
Tested Reactivity Hu
Predict Reactivity Dog, Rat, Pig
Tested Application WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name FTO
Antigen Species Mouse
Immunogen QQKPDCRPYWEKDD
Conjugation Un-conjugated
Alternate Names EC 1.14.11.-; Alpha-ketoglutarate-dependent dioxygenase FTO; Fat mass and obesity-associated protein; ALKBH9

Application Instructions

Application Suggestion
Tested Application Dilution
WB0.03 - 0.1 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 79068 Human FTO

Swiss-port # Q9C0B1 Human Alpha-ketoglutarate-dependent dioxygenase FTO

Gene Symbol Fto
Gene Full Name fat mass and obesity associated
Background This gene is a nuclear protein of the AlkB related non-haem iron and 2-oxoglutarate-dependent oxygenase superfamily but the exact physiological function of this gene is not known. Other non-heme iron enzymes function to reverse alkylated DNA and RNA damage by oxidative demethylation. Studies in mice and humans indicate a role in nervous and cardiovascular systems and a strong association with body mass index, obesity risk, and type 2 diabetes. [provided by RefSeq, Jul 2011]
Function Dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single-stranded RNA containing 3-methyluracil, followed by single-stranded DNA containing 3-methylthymine. Has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. Specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. Has no activity towards 1-methylguanine. Has no detectable activity towards double-stranded DNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Contributes to the regulation of the global metabolic rate, energy expenditure and energy homeostasis. Contributes to the regulation of body size and body fat accumulation. [UniProt]
Research Area Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody
Calculated MW 58 kDa