ARG23726

anti-FZD4 / Frizzled 4 antibody

anti-FZD4 / Frizzled 4 antibody for Western blot and Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes FZD4 / Frizzled 4
Tested Reactivity Ms
Predict Reactivity Hu
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name FZD4 / Frizzled 4
Antigen Species Human
Immunogen KLH-conjuated synthetic peptide around aa. 100-200 of Human Frizzled 4.
Conjugation Un-conjugated
Alternate Names Fz4; FzE4; CD344; CD antigen CD344; Frizzled-4; Fz-4; hFz4; GPCR; FZD4S; FEVR; EVR1

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol.
Preservative 0.09% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 14366 Mouse FZD4

Swiss-port # Q61088 Mouse Frizzled-4

Gene Symbol FZD4
Gene Full Name frizzled class receptor 4
Background This gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. This protein may play a role as a positive regulator of the Wingless type MMTV integration site signaling pathway. A transcript variant retaining intronic sequence and encoding a shorter isoform has been described, however, its expression is not supported by other experimental evidence. [provided by RefSeq, Jul 2008]
Function Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin (CTNNB1) canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin (CTNNB1) and activation of Wnt target genes. Plays a critical role in retinal vascularization by acting as a receptor for Wnt proteins and norrin (NDP). In retina, it can be both activated by Wnt protein-binding, but also by a Wnt-independent signaling via binding of norrin (NDP), promoting in both cases beta-catenin (CTNNB1) accumulation and stimulation of LEF/TCF-mediated transcriptional programs. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. [UniProt]
Calculated MW 60 kDa
PTM Ubiquitinated by ZNRF3, leading to its degradation by the proteasome. [UniProt]