ARG66638

anti-FZD9 / Frizzled 9 antibody

anti-FZD9 / Frizzled 9 antibody for Western blot,ICC/IF and Human,Monkey

Overview

Product Description Rabbit Polyclonal antibody recognizes FZD9 / Frizzled 9
Tested Reactivity Hu, Mk
Tested Application ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name FZD9 / Frizzled 9
Antigen Species Human
Immunogen Synthetic peptide between aa. 520-600 of Human FZD9 / Frizzled 9.
Conjugation Un-conjugated
Alternate Names Frizzled-9; Fz-9; FZD3; CD349; FzE6; CD antigen CD349; hFz9

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:200 - 1:1000
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~ 65 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol and 0.5% BSA
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 8326 Human FZD9

Swiss-port # O00144 Human Frizzled-9

Gene Symbol FZD9
Gene Full Name frizzled class receptor 9
Background Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD9 gene is located within the Williams syndrome common deletion region of chromosome 7, and heterozygous deletion of the FZD9 gene may contribute to the Williams syndrome phenotype. FZD9 is expressed predominantly in brain, testis, eye, skeletal muscle, and kidney. [provided by RefSeq, Jul 2008]
Function Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. [UniProt]
Cellular Localization Cell membrane; Multi-pass membrane protein. Note=Relocalizes DVL1 to the cell membrane leading to phosphorylation of DVL1 and AXIN1 relocalization to the cell membrane. [UniProt]
Calculated MW 64 kDa
PTM Ubiquitinated by ZNRF3, leading to its degradation by the proteasome. [UniProt]