ARG22573
anti-Factor VIII antibody [RFF-VIIIC/8]
anti-Factor VIII antibody [RFF-VIIIC/8] for ELISA,Radioimmunoassay,Western blot and Human,Pig
Overview
| Product Description | Mouse Monoclonal antibody [RFF-VIIIC/8] recognizes Factor VIII |
|---|---|
| Tested Reactivity | Hu, Pig |
| Species Does Not React With | Ms, Rat, Dog |
| Tested Application | ELISA, RIA, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | RFF-VIIIC/8 |
| Isotype | IgG1 |
| Target Name | Factor VIII |
| Antigen Species | Human |
| Immunogen | Affinity purified human Factor VIII. |
| Conjugation | Un-conjugated |
| Alternate Names | AHF; Antihemophilic factor; Coagulation factor VIII; HEMA; F8B; F8C; Procoagulant component; FVIII; DXS1253E |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% Sodium azide |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | F8 |
| Gene Full Name | coagulation factor VIII, procoagulant component |
| Background | This gene encodes coagulation factor VIII, which participates in the intrinsic pathway of blood coagulation; factor VIII is a cofactor for factor IXa which, in the presence of Ca+2 and phospholipids, converts factor X to the activated form Xa. This gene produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein, isoform a, which circulates in plasma and associates with von Willebrand factor in a noncovalent complex. This protein undergoes multiple cleavage events. Transcript variant 2 encodes a putative small protein, isoform b, which consists primarily of the phospholipid binding domain of factor VIIIc. This binding domain is essential for coagulant activity. Defects in this gene results in hemophilia A, a common recessive X-linked coagulation disorder. [provided by RefSeq, Jul 2008] |
| Function | Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa. [UniProt] |
| Calculated MW | 267 kDa |
| PTM | Sulfation on Tyr-1699 is essential for binding vWF. |
