ARG22573

anti-Factor VIII antibody [RFF-VIIIC/8]

anti-Factor VIII antibody [RFF-VIIIC/8] for ELISA,Radioimmunoassay,Western blot and Human,Pig

Overview

Product Description Mouse Monoclonal antibody [RFF-VIIIC/8] recognizes Factor VIII
Tested Reactivity Hu, Pig
Species Does Not React With Ms, Rat, Dog
Tested Application ELISA, RIA, WB
Host Mouse
Clonality Monoclonal
Clone RFF-VIIIC/8
Isotype IgG1
Target Name Factor VIII
Antigen Species Human
Immunogen Affinity purified human Factor VIII.
Conjugation Un-conjugated
Alternate Names AHF; Antihemophilic factor; Coagulation factor VIII; HEMA; F8B; F8C; Procoagulant component; FVIII; DXS1253E

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA1:500 - 1:15000
RIAAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS and 0.09% Sodium azide
Preservative 0.09% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2157 Human F8

GeneID: 397339 Pig F8

Swiss-port # P00451 Human Coagulation factor VIII

Swiss-port # P12263 Pig Coagulation factor VIII

Gene Symbol F8
Gene Full Name coagulation factor VIII, procoagulant component
Background This gene encodes coagulation factor VIII, which participates in the intrinsic pathway of blood coagulation; factor VIII is a cofactor for factor IXa which, in the presence of Ca+2 and phospholipids, converts factor X to the activated form Xa. This gene produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein, isoform a, which circulates in plasma and associates with von Willebrand factor in a noncovalent complex. This protein undergoes multiple cleavage events. Transcript variant 2 encodes a putative small protein, isoform b, which consists primarily of the phospholipid binding domain of factor VIIIc. This binding domain is essential for coagulant activity. Defects in this gene results in hemophilia A, a common recessive X-linked coagulation disorder. [provided by RefSeq, Jul 2008]
Function Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa. [UniProt]
Calculated MW 267 kDa
PTM Sulfation on Tyr-1699 is essential for binding vWF.