ARG64408

anti-Factor XIIIa antibody

anti-Factor XIIIa antibody for Western blot and Human

Cell Biology and Cellular Response antibody; Controls and Markers antibody

Overview

Product Description Goat Polyclonal antibody recognizes Factor XIIIa
Tested Reactivity Hu
Predict Reactivity Ms
Tested Application WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name Factor XIIIa
Antigen Species Human
Immunogen C-HRKLIASMSSDSLRH
Conjugation Un-conjugated
Alternate Names Coagulation factor XIIIa; F13A; Protein-glutamine gamma-glutamyltransferase A chain; Coagulation factor XIII A chain; Transglutaminase A chain; EC 2.3.2.13

Application Instructions

Application Suggestion
Tested Application Dilution
WB0.05 - 0.2 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2162 Human F13A1

Swiss-port # P00488 Human Coagulation factor XIII A chain

Background This gene encodes the coagulation factor XIII A subunit. Coagulation factor XIII is the last zymogen to become activated in the blood coagulation cascade. Plasma factor XIII is a heterotetramer composed of 2 A subunits and 2 B subunits. The A subunits have catalytic function, and the B subunits do not have enzymatic activity and may serve as plasma carrier molecules. Platelet factor XIII is comprised only of 2 A subunits, which are identical to those of plasma origin. Upon cleavage of the activation peptide by thrombin and in the presence of calcium ion, the plasma factor XIII dissociates its B subunits and yields the same active enzyme, factor XIIIa, as platelet factor XIII. This enzyme acts as a transglutaminase to catalyze the formation of gamma-glutamyl-epsilon-lysine crosslinking between fibrin molecules, thus stabilizing the fibrin clot. It also crosslinks alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin. Factor XIII deficiency is classified into two categories: type I deficiency, characterized by the lack of both the A and B subunits; and type II deficiency, characterized by the lack of the A subunit alone. These defects can result in a lifelong bleeding tendency, defective wound healing, and habitual abortion. [provided by RefSeq, Jul 2008]
Research Area Cell Biology and Cellular Response antibody; Controls and Markers antibody
Calculated MW 83 kDa
PTM The activation peptide is released by thrombin.