ARG23130

anti-FeLV p27 antibody [PF12J-10A]

anti-FeLV p27 antibody [PF12J-10A] for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Virus

Overview

Product Description Mouse Monoclonal antibody [PF12J-10A] recognizes FeLV p27
Mouse anti Feline Leukaemia Virus p27 antibody, clone PF12J-10A recognises feline leukaemia virus (FeLV) p27 core protein (p27).The oncoretrovirus FeLV is responsible for both neoplastic and non-neoplastic conditions, often resulting in a poor prognosis and fatal outcome for cats persistently infected. During persistent infection the virus, soluble viral antigens and antigen containing white blood cells are continuously present in the blood and diagnosis is mainly based upon detection of the p27 antigen - using ELISA to test for p27 as it circulates free in the bloodstream, and Immunofluorescence studies to detect p27 within the infected white cells.
Tested Reactivity Virus
Tested Application ELISA, IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone PF12J-10A
Isotype IgG1
Target Name FeLV p27
Antigen Species Virus
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
IHC-P1:100
WB1:1500
Application Note WB: This antibody detects a band of approximately 28 kDa under reducing conditions. A strong band of 80 kDa representing glycosylated FeLV gag gene product may also be present.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS and 0.05% Sodium azide.
Preservative 0.05% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.