ARG22645
anti-Fibroblasts + Epithelial cells antibody [D7-FIB]
anti-Fibroblasts + Epithelial cells antibody [D7-FIB] for Flow cytometry,ICC/IF,IHC-Frozen sections,Immunoprecipitation and Human
Overview
| Product Description | Mouse Monoclonal antibody [D7-FIB] recognizes Fibroblasts + Epithelial cells This antibody recognizes a ~112kDa molecule expressed on the cell surface of Human fibroblasts. The antigen is not expressed by peripheral blood cells, and is found at low levels on a minority of melanoma cell lines. Studies upon the antigen have shown it to be sensitive to SDS, but resistant to trypsin, tunicamycin and collagenase. In immunohistological studies Mouse anti Human Fibroblasts/Epithelial Cells antibody. Mouse anti Human Fibroblasts/Epithelial Cells antibody, clone D7-FIB has also been found to bind to epithelium, myoepthelium, smooth muscle and some leucocytes. Mouse anti Human Fibroblasts/Epithelial Cells antibody, clone D7-FIB is useful as a cell membrane marker to characterize chondrocyte differentiation giving a positive reaction with dedifferentiated Human chondrocytes, and negative with differentiated chondrocytes (van Osch et al. 2001). Mouse anti Human Fibroblasts/Epithelial Cells antibody, clone D7-FIB is routinely tested in flow cytometry on the KG1 cell line. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, IHC-Fr, IP |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | D7-FIB |
| Isotype | IgG2a |
| Target Name | Fibroblasts + Epithelial cells |
| Antigen Species | Human |
| Immunogen | Human foreskin fibroblasts. |
| Conjugation | Un-conjugated |
Application Instructions
| Application Suggestion |
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| Application Note | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% Sodium azide. |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
