ARG53974
anti-Fibronectin antibody
anti-Fibronectin antibody for IHC-Formalin-fixed paraffin-embedded sections and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Fibronectin |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P |
| Specificity | This antibody reacts with human fibronectin in liver, tonsil, skin and kidney. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Fibronectin |
| Antigen Species | Human |
| Immunogen | Purified human Fibronectin from human plasma. |
| Conjugation | Un-conjugated |
| Alternate Names | ED-B; CIG; GFND; Cold-insoluble globulin; FNZ; LETS; GFND2; Fibronectin; MSF; FINC; FN |
Application Instructions
| Application Note | Immunohistochemistry (IHC) 1:100-1:400 for 30 minute at room temp by polymer system or streptavidin-biotin system. Formalin-fixed paraffin-embedded (FFPE) tissue sections and cell smears require antigen retrieval by boiling tissue in 10 mM citrate buffer, pH 6.0 for 10-20 minute, followed by cooling at room temp. for 20 minutes. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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Properties
| Form | Liquid |
|---|---|
| Purification Note | Purified antibody. |
| Buffer | 10 mM PO4 (pH 7.4), 150 mM NaCl and 0.05% Sodium azide. |
| Preservative | 0.05% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | FN1 |
| Gene Full Name | fibronectin 1 |
| Background | Fibronectin is a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and extracellular matrix. Fibronectin is involved in cell adhesion and migration process including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. They also occur as dimmer of two 200 kDa subunits. Fibronectin has been implicated in carcinoma development in lung cancer. Fibronectin expression is increased especially in non-small cell lung carcinoma. The adhesion of lung carcinoma cells to Fibronectin enhances tumorgenecity and confers resistance to apoptosis induced by standard chemotherapeutic agents. |
| Function | Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts. Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. [UniProt] |
| Cellular Localization | Secreted › extracellular space › extracellular matrix. Signal will show in connective tissue matrix and basement membrane. |
| Highlight | Related products: Fibronectin antibodies; Fibronectin ELISA Kits; Fibronectin Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: New antibody panels for Myofibroblasts and CAFs |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Signaling Transduction antibody |
| Calculated MW | 272 kDa |
| PTM | Sulfated. It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated. Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers). Phosphorylated by FAM20C in the extracellular medium. Proteolytic processing produces the C-terminal NC1 peptide, anastellin. Some lysine residues are oxidized to allysine by LOXL3, promoting fibronectin activation and matrix formation. |
