ARG66162

anti-Fibronectin antibody

anti-Fibronectin antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

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Overview

Product Description

Mouse Monoclonal antibody recognizes Fibronectin

Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, WB
Host Mouse
Clonality Monoclonal
Isotype IgG
Target Name Fibronectin
Antigen Species Human
Immunogen Synthetic peptide from Human Fibronectin
Conjugation Un-conjugated
Alternate Names ED-B; CIG; GFND; Cold-insoluble globulin; FNZ; LETS; GFND2; Fibronectin; MSF; FINC; FN

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:300
WB1:1000 - 1:2000
Application Note IHC-P: Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 14268 Mouse FN1

GeneID: 2335 Human FN1

Swiss-port # P02751 Human Fibronectin

Swiss-port # P11276 Mouse Fibronectin

Gene Symbol FN1
Gene Full Name fibronectin 1
Background This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]
Function Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.

Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. [UniProt]
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Calculated MW 272 kDa
PTM Sulfated.
It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
Phosphorylated by FAM20C in the extracellular medium.
Proteolytic processing produces the C-terminal NC1 peptide, anastellin.
Some lysine residues are oxidized to allysine by LOXL3, promoting fibronectin activation and matrix formation.

Customer's Feedback

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Review for anti-Fibronectin antibody

Application:WB

Sample:Huh7 and Huh7 shHMGB1

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

Anticancer Effects and Molecular Mechanisms of Apigenin in Cervical Cancer Cells

WB, IHC-P / Human

Ya-Hui Chen et al.
Cancers (Basel).,  (2022)

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